Competition between Serum IgG, IgM, and IgA Anti-Glycan Antibodies
Fig 2
Dose-dependent inhibition of IgG and IgA signals upon addition of IgM.
(A) Decrease in IgG signals with increase in IgM concentration. (B) Decrease in IgA signals with increase in IgM concentration. Polyclonal IgG and IgA isolated from serum were first profiled on the array alone. Separately, they were pre-mixed with varying amounts of IgM and then profiled on the array. The box plots depict the range of IgG or IgA signals (on a log base 2 scale) alone or in the presence of varying amounts of IgM (50–400 μg/mL). The line in the middle of the box is the median signal, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. The box plots demonstrate decreasing IgG and IgA signals in the presence of increasing amounts of IgM across the entire array.