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Analysis of Genetic Code Ambiguity Arising from Nematode-Specific Misacylated tRNAs

Figure 2

Subcellular localization of nev-tRNAs in C. elegans.

RNA was isolated from each fraction of C. elegans: whole cell (W), nuclear (N), or cytoplasmic (C). RT–PCR analysis was used to detect snU6 and snoU3 RNAs (nuclear markers), tRNAiMet (cytoplasmic marker), and four tRNAs (nev-tRNAGly and nev-tRNAIle, and their cognate tRNAs). 5S rRNA expression is shown as the loading control. Band densities were evaluated semiquantitatively with densitometry.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0116981.g002