Analysis of Genetic Code Ambiguity Arising from Nematode-Specific Misacylated tRNAs
RNA was isolated from each fraction of C. elegans: whole cell (W), nuclear (N), or cytoplasmic (C). RT–PCR analysis was used to detect snU6 and snoU3 RNAs (nuclear markers), tRNAiMet (cytoplasmic marker), and four tRNAs (nev-tRNAGly and nev-tRNAIle, and their cognate tRNAs). 5S rRNA expression is shown as the loading control. Band densities were evaluated semiquantitatively with densitometry.