Advancements in the Development of HIF-1α-Activated Protein Switches for Use in Enzyme Prodrug Therapy
(A) Diagrams of RICP12 and RICP95 sequences identified from the random circular permutation and random insertion library. Numbers above indicate the amino acid residues of the corresponding wild-type proteins. Letters below indicate the 1-letter abbreviation of the amino acid linker sequence used in the circular permutation of the CH1 domain. (B) Dot toxicity assay of RICP12 and RICP95.