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A Fully Automated Microfluidic Femtosecond Laser Axotomy Platform for Nerve Regeneration Studies in C. elegans

Figure 8

Imaging of regrowing axons of interest 24 hours after surgery.

a) Images at three different z-locations showing an example of reconnection with the connection point of distal and proximal ends indicated by the arrow. b) Images at three different focal planes showing regrowth with a lack of reconnection, as evidenced by the severed ends taking paths in different focal planes. The fragmentation and beading in the distal part shows the lack of reconnection.

Figure 8

doi: https://doi.org/10.1371/journal.pone.0113917.g008