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Melanin Photosensitization and the Effect of Visible Light on Epithelial Cells

Figure 5

Melanin photosensitization with visible light damages nuclear DNA.

(Left) Comet assay. (A) B16-F10 CT cells in the dark after treatment with 0.2 U of Fpg (treatment with Endo III yielded the same results); (B) B16-F10 M++++ cells in the dark after treatment with 0.2 U of Fpg (treatment with Endo III yielded the same results); (C) B16-F10 CT cells exposed to 6 J.cm−2 of visible irradiation after treatment with 0.2 U of Fpg (treatment with Endo III yielded the same results); (D) B16-F10 M++++ cells exposed to 6 J.cm−2 of visible irradiation without enzymes; (E, F) the same as D treated with 0.2 U of Endo III (E) and Fpg (F). The graphic on the left shows the quantification of the DNA fragmentation in B16-F10 cells under conditions A through E. (Right) A schematic of melanin photosensitization via type I and type II mechanisms, leading to damage in several biological targets, including the cell membrane and nuclear DNA. The DNA changes are indicated by using the main Fpg and Endo III recognition sites.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0113266.g005