A Simple Isothermal DNA Amplification Method to Screen Black Flies for Onchocerca volvulus Infection
Figure 5
Sensitivity of LAMP and PCR methods for the detection of O. volvulus using ten-fold serial dilutions of O. volvulus genomic DNA ranging from 0.001–1.0 ng.
Detection of LAMP product using turbidity (A) or hydroxy napthol blue (B). PCR amplification of a ∼200 bp product using LAMP primers F3 and B3 was obtained when O. volvulus genomic DNA was used (C). Molecular weight marker (MW) is indicated.