Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Intra-Lesional Injection of the Novel PKC Activator EBC-46 Rapidly Ablates Tumors in Mouse Models

Figure 4

EBC-46 anti-cancer efficacy is PKC-dependent.

A. The effect of neutrophil depletion on EBC-46 treatment of SK-MEL-28 tumors grown on BALB/c Foxn1nu mice. Tumor growth in mice given anti-Ly-6G antibody, isotype control antibody or no antibody. SK-MEL-28 cells were injected sub-cutaneously (n = 10 tumors/group; 2 tumors/mouse, 5 mice/group) and allowed to reach approximately 100 mm3. Mice were injected with anti-Ly-6G antibody (clone 1A8; 100 µg i.p, on days −2, 0, and 2), with isotype control antibody (IgG2a, clone 2A3; 100 µg i.p, on days −2, 0, and 2) or with nothing. Tumors were treated by intra-lesional injection of vehicle (50 µl of 20% propylene glycol in water) on day 0. The tumor volumes represent the mean volume of individual tumors. • – no antibody, ▪ – control IgG2a antibody, ▴ – anti-mLy-6G antibody. B. Tumor growth in mice given anti-Ly-6G antibody, isotype control antibody or no antibody after EBC-46 treatment. As for A, SK-MEL-28 tumors (approximately 100 mm3) were treated by intra-lesional injection with 25 nmol (15 µg) EBC-46 (in 50 µl of 20% propylene glycol in water) on day 0. • – no antibody, ▪ – control IgG2a antibody, ▴ – anti-mLy-6G antibody. Error bars – SD. C. 500,000 B16-F0 cells were injected into BALB/c Foxn1nu mice, and allowed to reach >50 mm3. Tumors were then treated with either 50 µl vehicle (20% propylene glycol in water), 16.7 nmol (10 µg) EBC-46 in vehicle or 16.7 nmol (10 µg) EBC-46 after pre-treatment with 5 µM bisindolylmaleimide-1 (BIS-1) in vehicle. Tumor size was measured 8 days after treatment. Error bars = SD, n = 12. (****, p<0.0001; **, p = 0.0024; t-test). D. Number of tumors (as percentage) present 8 days after treatment. Total tumor number n = 12 for each group.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0108887.g004