Adaptive Evolution of Synthetic Cooperating Communities Improves Growth Performance
The model was constrained using either amino acid uptake (panels A and C) or release rates (panels B and D). Panels A and B display the predicted K:L ratio at a co-culture OD≈0.2. The color map indicates the value of K:L ratio. Panels C and D show the predicted average growth rate of co-culture, indicated by the color map. The evolutionary trajectory of co-culture 4 is shown on panels A through D, where the open circles indicate passages 1,4,7,10,12,15,19 and 21. The estimated uptake or release rates for evolved Kev and Lev strains in each passage were then used to constrain the model. Panel E compares the model predicted K:L ratio and average growth rate of the co-culture near OD600≈0.2 to the estimated experimental values. Blue diamonds and red triangles denote the predictions when the model was constrained by the estimated uptake rates for co-culture 4 and 6, respectively. Green squares and purple circles denote the predictions when the model was constrained by the estimated release rates for the two co-cultures for co-culture 4 and 6, respectively.