Adaptive Evolution of Synthetic Cooperating Communities Improves Growth Performance
Cells from 10 colonies of Kev (or Lev) at each selected passage were grown individually in co-culture with the un-evolved partner strain (L or K). The growth rate and change in OD600 for each hybrid co-culture was normalized to the growth rate and change in OD600 of the un-evolved co-culture grown on the same microplate. The resulting growth rate ratios and change in OD600 ratios are shown as blue diamonds (L+Kev) and red squares (Lev+K), respectively, in panels A and B (isolates from co-culture 4) and panels D and E (isolates from co-culture 6). The error bars indicate the standard deviations based on 10 separate hybrid co-cultures each with four replicates (n = 40). The dashed lines indicate the behavior of the un-evolved co-culture (L+K). Panels C and F shows the K:L ratio in L+Kev and Lev+K in hybrid co-cultures and the un-evolved co-culture. The hybrid co-cultures contained evolved isolates from co-culture 4 (panel C) or co-culture 6 (panel F). The error bars indicate the standard deviations based on hybrid co-cultures using three different isolates and three measurements for each passage (n = 9), see File S2 for details. The shaded bands in C and F show the mean ± the standard deviation for the K:L ratio in the un-evolved co-culture at an OD600 of 0.2 when grown in 96 well plates (1.62±0.14).