Adaptive Evolution of Synthetic Cooperating Communities Improves Growth Performance
Three randomly selected colonies of Kev (or Lev) from passage 19 of co-culture 4 and passage 18 of co-culture 6 were inoculated in glucose minimal medium with various amounts of lysine (for Kev strains, panel A) or leucine (for Lev strains, panel B). Each colony was tested in three replicate mono-cultures. The growth rates were calculated for the evolved and un-evolved parental strains (control). The error bars represent the standard deviations across biological replicates.