Genetic and Phenotypic Comparison of Facultative Methylotrophy between Methylobacterium extorquens Strains PA1 and AM1
Figure 5
Comparison of growth rates and maximum OD600 values on methanol with succinate versus on succinate alone.
A) Ratio of growth rates, of the Δcel ‘wildtype’ strain of PA1 and various knockout mutants, on a combination of ½M+½S (7.5 mM methanol+1.75 mM succinate) versus S (3.5 mM succinate). The dotted line depicts the expected ratio for growth rate, if no methanol was oxidized in a combination of M+S. B) Ratio of yield (measured as the maximum OD600 value during growth), for the Δcel ‘wildtype’ strain of PA1 and various knockout mutants, on M+S versus S. The dotted line depicts expected ratio for yields, if no methanol was assimilated in a combination of M+S. For all data error bars represent the 95% C.I. of the average ratio of three biological replicates grown in each condition.