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How Do Haloarchaea Synthesize Aromatic Amino Acids?

Figure 10

Genomic contexts of ORFs involved AroAA biosynthesis in H. salinarum.

Arrows show the relative positions and orientations of ORFs (but are not drawn to scale); those colored in red, green, blue or yellow represent ORFs assigned to the AroAA biosynthesis pathway while other, nearby ORFs, are uncoloured. A and B, ORFs assigned to convert precursors to DHQ. The first and second ORFs (OE1472F in red and OE1475F in green) are homologs of M. jannaschii ORFs [6]. These form part of the non-canonical part of the pathway. ORF OE1477R (in blue) is the 3rd ORF discussed in this paper. C and D, ORFs needed to convert DHQ to chorismate. F and G, the tryptophan branch. H, the tyrosine branch, I-the phenylalanine branch. J- the genomic context of ORF OE2019F, homolog to OE1472F. The numbers below the arrows represent the induction of AroAA-related genes in H. salinarum R1 cells grown in synthetic medium without AroAA relative to synthetic medium with AroAA. Induction values ≥2.0 are marked in red, and have p values of ≤10−3 unless indicated. * The two ORFs are involved in the conversion of chorismate to para-aminobenzoate, an intermediate of folate biosynthesis, NS, not significant; ND, not detected; CHY, conserved hypothetical protein; HY, hypothetical protein. ORF names and predicted functions are derived from the genome annotation at www.halolex.mpg.de.

Figure 10

doi: https://doi.org/10.1371/journal.pone.0107475.g010