How Do Haloarchaea Synthesize Aromatic Amino Acids?
P1,P2 and P3 and their associated arrows indicate the relative positions and orientations of the primers used for PCR. After in-frame integration of plasmid pMG501, PCR was expected to generate a product in transformed cells but not for the WT. A, Illustration of WT H. salinarum genotype. B, illustration of the mutant genotype after incorporation of plasmid pMG501. C and D, Agarose gel electrophoresis of PCR products using primers P1 and P2, and primers P3 and P2, respectively. Lane 1: MW markers (in bp), lane2: Chromosomal DNA of R1, lane 3: plasmid pMG501 (control), lanes 4–6- transformed colonies. E, Relative expression of OE1472F transcription in mutant StopOE1472F (OE1471F::pMG501) grown in synthetic medium without AroAA to OD600nm = 0.4 and 1.0. Results were obtained using RT-qPCR. See table S7 in file S1 for details of the primers used.