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Fluorescence2D: Software for Accelerated Acquisition and Analysis of Two-Dimensional Fluorescence Spectra

Figure 1

Rectangular and triangular two-dimensional spectra.

(A) Example of a conventional array of excitation/emission wavelengths used for acquisition of two-dimensional fluorescence spectra. The diagonal (λem = λex) is shown with the open symbols. The conventional 1D emission spectrum is a horizontal set of points for each excitation wavelength to the right of the open symbol. Correspondingly, the 1D excitation spectrum is a vertical set of points below the open symbol. (B) The triangular array of excitation/emission wavelengths where the emission ranges always begin at a greater wavelength than the excitation wavelength in each row. The specific 100 nm increment in this figure is chosen solely for clarity of presentation.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0101227.g001