Molecular Hydrogen Attenuates Neuropathic Pain in Mice
Figure 5
Hydrogen suppressed the development of oxidative stress in the spinal cord dorsal horn.
(A–F) Representative images of immunohistochemical staining for the oxidative stress markers 4-HNE (A–C) and 8-OHdG (D-F) in the spinal cord dorsal horn at the level of L5. Staining for 4-HNE was increased in the spinal cord at the end of 4 days period after PSNL (A) compared with sham operation (C). (B) The number of 4-HNE positive cells was decreased in mice with hydrogen when compared with the H2 (–) group (A). Similarly, staining for 8-OHdG was also increased in the spinal cord from mice with PSNL (D) compared with sham operation (F). (E) The number of 8-OHdG positive cells was decreased in mice with hydrogen at the end of 4 days period after PSNL when compared with the H2 (–) group (D). (G, H) The numbers of positive cells for both markers were significantly decreased when compared with the H2 (–) group. *P<0.05 compared with control (t test, n = 6 mice for each). Scale bar: 200 µm.