Elimination of Young Erythrocytes from Blood Circulation and Altered Erythropoietic Patterns during Paraquat Induced Anemic Phase in Mice
Mice were treated with paraquat and spleen cells were harvested as described in legends of Figure 4. Spleen cells were stained with anti-mouse Ter 119-APC and anti-mouse CD71-PE antibodies followed by flow cytometric analysis to delineate four distinct stages (p, q, r and s) in erythroid differentiation as described in the text. Values in parentheses next to the gated cells represent the percentage values of different subpopulations of erythroid as percentage of all spleen cells Panels A and B illustrate typical gating of for the four stages (p, q, r and s) in spleen cells from control and paraquat treated mice respectively. Panels C, D, E and F illustrate the kinetics of changes in proportions of proerythroblast (population-p), erythroblast A (population-q), erythroblast B (population-r) and erythroblast C (population s) respectively in the spleens of control (○) and paraquat treated (•) mice. Data represents mean ± SEM; n = 6 in each groups. *p<0.05, **p<0.005. Values in parentheses represent percentage of erythroid cells.