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Elimination of Young Erythrocytes from Blood Circulation and Altered Erythropoietic Patterns during Paraquat Induced Anemic Phase in Mice

Figure 5

Time kinetics of paraquat induced changes in the proportions of cells in different stages of erythroid differentiation in bone marrow.

Mice were treated with paraquat and bone marrow cells were harvested as described in legends of Figure 4. Bone marrow cells were stained with anti-mouse Ter 119-APC and anti-mouse CD71-PE antibodies followed by flow cytometric analysis to delineate four distinct stages (p, q, r and s) in erythroid differentiation as described in the text. Panels A and B illustrate typical gating of for the four stages (p, q, r and s) in bone marrows from control and paraquat treated mice respectively. Values in parentheses next to the gated cells represent the percentage values of different subpopulations of erythroid as percentage of all bone marrow cells. Panels C, D, E and F illustrate the kinetics of changes in proportions of proerythroblast (population-p), erythroblast A (population-q), erythroblast B (population-r) and erythroblast C (population s) respectively in bone marrows of control (○) and paraquat treated (•) mice. Data represents mean ± SEM; n = 6 in each groups. *p<0.05, **p<0.005 and ***p<0.0005.

Figure 5