Elimination of Young Erythrocytes from Blood Circulation and Altered Erythropoietic Patterns during Paraquat Induced Anemic Phase in Mice
Gating of erythroid cells in bone marrow and spleen cell preparations and time dependent changes in relative proportions and absolute recoveries of erythroid cells in bone marrows and spleens of control and paraquat treated mice.
Mice were treated with repeated doses of paraquat (10 mg/kg, i.p) on alternate days. Bone marrow and spleen cells were harvested just before the beginning of paraquat treatment and at different time points thereafter. Proportion of erythroid cells in bone marrow and spleen was determined by staining with anti-mouse Ter 119-APC and anti-mouse CD71-PE antibody and enumeration of cells falling within the inverted L-shaped gate as described in methods. Panels A, B, C and D show representative flow cytometric histograms depicting the inverted L shaped gates that defined the erythroid populations in bone marrow (panels A, B) and spleens (panels C, D) of control (left panels) and paraquat treated (right panels) mice. Values in parentheses next to the gated cells represent erythroid cells as percentage of all bone marrow (panel A, B) or spleen cells (panels C, D). Panels E, and F show summarized data of time dependent changes in the proportions (panel E) and absolute recoveries (panel F) of erythroid cells in bone marrow of control (○) and paraquat treated (•) mice. Similar data for spleen cells is shown in panels G and H). Data in panels E to H represents mean ± SEM; n = 6 in each groups. *p<0.05, **p<0.005 and ***p<0.0005.