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Analysis of the Central Nervous System Transcriptome of the Eastern Rock Lobster Sagmariasus verreauxi Reveals Its Putative Neuropeptidome

Figure 7

Crustacean hyperglycemic hormone (CHH) precursors predicted complete and partial ORFs with high similarity levels.

A–E) Complete and partial CHH isoforms 1–5 (derived from CL7809.Contig1_All, CL7809.Contig3_All, CL7809.Contig4_All, Unigene30324_All and Unigene34312_All) with signal peptides (red) and predicted CHH domains (green) with an amidated glycine (light blue), preceded by carboxyl-peptidase cleavage sites (underlined). Six conserved cysteine residues predicted to form 3 disulfide bridges are highlighted in yellow. Asterisk indicates the stop codon. F) CHH domains conservation: the 71–73 aa domains show high level of similarity with each other. G) CHH domains phylogenetic tree showing similarity levels are highest between isoforms B1 and B2, followed by isoform B4, then isoform B3 and furthest is the unspecified CHH isoform. Scale bar represents number of substitutions per site.

Figure 7