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Phosphatidylinositol-4,5-Bisphosphate Enhances Anionic Lipid Demixing by the C2 Domain of PKCα

Figure 6

²H-NMR representative spectra obtained at the temperatures shown.

(A) POPC/POPC-d₃₁ (2.5∶1, molar ratio); (B) POPC/POPC-d₃₁ (2.5∶1, molar ratio) in the presence of C2α domain (40∶1 phospholipid/protein molar ratio); (C) POPS/POPS d₃₁ (3∶1 molar ratio) and (D) POPS/POPS d₃₁ (3∶1 molar ratio) in the presence of C2α domain (40∶1 phospholipid/protein molar ratio).

doi: https://doi.org/10.1371/journal.pone.0095973.g006