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Exposure to Bisphenol A Correlates with Early-Onset Prostate Cancer and Promotes Centrosome Amplification and Anchorage-Independent Growth In Vitro

Figure 3

An increase in centrosome numbers is seen in prostate cancer cells exposed to BPA.

(I) An increase in centrosome numbers. The cell lines NPrEC, RWPE1, LNCaP, C4-2, 22Rv1 and PC3 were treated with medium containing 10% CSS plus 0 or 0.1 nM BPA for 72 h. Cells were fixed with 100% cold methanol and immunostained for centrosomes (anti-γ-tubulin, red) and nucleus (DAPI, blue). The cells were examined by fluorescence microscopy. Arrows point to the positions of centrosomes, and panels on the right show magnified images of the indicated areas. Scale bar, 10 µm. (II) Centrosome amplification in the presence of BPA is not due to centriole separation. RWPE-1 cells were treated with 0.1 nM BPA for 3 days. Cells were fixed and immunostained for centrosomes (anti-γ-tubulin, red), centrioles (anti-centrin, green), and nucleus (DAPI, blue). Arrows point to the positions of centrosomes. Panels on right show magnified images of the indicated areas. Scale bar, 10 µm.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0090332.g003