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Probiotic Microbes Sustain Youthful Serum Testosterone Levels and Testicular Size in Aging Mice

Figure 2

Dietary L. reuteri increases the cross-sectional profile area of seminiferous tubules and their germ cell population.

a. Representative histology of seminiferous tubules cross-sectioned profiles of Swiss mice at the age of 5 months. Increased testicular weight due to L. reuteri consumption in both control- and New Western diet (NWD)-fed mice co-existed with a histologically discernible increase of seminiferous tubule (ST) cross-sectional area. Hematoxylin and eosin. Bars = 100 µm. b. Histomorphometrical assessment of circular ST cross-sectioned profiles size in different time points. The effect of L. reuteri remains highly significant with the progression of age. Numbers on the y axis of bar graphs correspond to the mean±SEM of ST area size; *** p<0.0001. c. Stereological morphometric assessment shows that L. reuteri-fed mouse testes have significantly increased nuclear volumes of germ cells. Numbers on the y axis of bar graphs correspond to the mean±SEM of “Nuclear volume of germ cells per unit area” or the “absolute nuclear volume of germ cells”; *p<0.05, **p<0.001, ***p<0.0001.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0084877.g002