Chemical Camouflage– A Frog's Strategy to Co-Exist with Aggressive Ants
Fractionation of the skin secretion from Phrynomantis microps by reversed-phase HPLC on a LiChroCART column, 125×4 mm (Merck, Darmstadt) which was eluted with a linear gradient from 0 to 60% acetonitrile in 0.1% trifluoroacetic acid (dotted line) over 60 min at a flow rate of 0.5 ml/min.
Absorbance was monitored at 220(above). Below: Total ion chromatogram (TIC) of liquid chromatography-time-of-flight mass spectrometry (LC/TOF MS) analysis of the HPLC fraction containing two peptides: m/z 1029.506 and m/z 1143.548 (non-protonated). De novo sequencing of the peptides suggested the tentative sequences as indicated in the chromatograms. Inlet picture: adult Phrynomantis microps examined by Paltothyreus tarsatus workers.