PCaAnalyser: A 2D-Image Analysis Based Module for Effective Determination of Prostate Cancer Progression in 3D Culture
Figure 10
The major steps involved in generating the classified read-map via boundary-mask and threshold-mask. Images depict a DU145 spheroid grown in a 3D matrix following immuno-labelling for the α6 integrin subunit. Panels in this figure refer to the intensity of the antibody and distribution of the α6 integrin subunit. Labelling was present primarily in the peripheral region of the spheroid structure.