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Oyster Larvae Settle in Response to Habitat-Associated Underwater Sounds

Figure 2

Larval settlement experiment (Laboratory) setup.

a) Side view schematic diagram of a replicate experimental tank (0.25 m×0.5 m) used in lab-based settlement experiment 1, showing the placement of a submerged speaker and container housing oyster larvae within a water bath, and b) Cylindrical treatment tank (20-L, 0.3 m water depth) used in lab experiment 2 containing an underwater speaker and five larval culture jars containing groups of actively swimming larvae. Larger view of larval culture jars with and without substrate is shown. No additional substrate was provided in experiment 1 and 2a; oyster shell discs were placed in the bottom of containers in experiment 2b.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0079337.g002