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The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

Figure 5

Accurate (green), inaccurate (red), and inhibited (blue) quantification of MAP-K10 in liver (A, n=36) and spleen (B, n=36) by TaqMan real-time PCR (MAP0865 gene), of M. tuberculosis H37Rv in lung (C, n=36) by TaqMan real-time PCR (ext-RD9 element), and of C57BL/6J mouse cells (D, n=36) by SyberGreen real-time PCR (PTGR2 gene), according to host DNA purity at 260/280 nm (on the left), host DNA quantity at 260 nm (on the right).

Figure 5