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The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

Figure 3

Comparison of MAP K-10 quantification methods in culture samples (A, n=28), or in liver (B, n=45) and spleen (C, n=45) samples from C57BL/6 mice: culture on 7H10-mycobactin versus TaqMan real-time PCR targeting MAP0865 gene, including 1 h, 24 h, or 48 h of lysozyme digestion (Ly), 1 or 2 phenol:chloroform:isoamyl purifications (PCI), no or 1 grinding step (G), and/or 0, 1, 2 or 3 steps of bead beating (BB) during DNA extraction procedure.

Error bars are standard deviation.

Figure 3