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The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

Figure 2

DNA amount measured at 260 nm after DNA extractions (a: van Soolingen procedure, b: Invisorb Spin Tissue Mini Kit from Invitek, c: PowerSoil DNA isolation Kit from MoBio) from big (+++: 10 log10 cells), medium (++: 9 log10 cells), and small (+: 8 log10 cells) pellets of MAP K-10 (A, n=54), and M. tuberculosis H37Ra (B, n=54) cells.

Error bars are standard deviation. LOQ are limit of quantification of the spectrophotometer.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0078749.g002