Identification of a Sex-Linked SNP Marker in the Salmon Louse (Lepeophtheirus salmonis) Using RAD Sequencing
Figure 2
Analysis of prohibitin-2 in male and female L. salmonis.
(A) Genotyping using the allele specific KASP assay. A total of 72 individuals (36 male and 36 female) from three unrelated L. salmonis strains (S, PT and FI) were genotyped using an allele specific PCR assay (KASPTM, LGC Genomics, UK). Individual L. salmonis genotype assignment was performed through reading the fluorescence emission of the FAM (Allele 1) and CAL Fluor Orange 560 (Allele 2) fluorophores for each sample, in comparison to no-template control reactions. The results of this PCR genotyping assay confirmed complete association of SNP genotype with L. salmonis sex as identified by RAD-seq analysis. (B) Differential expression of prohibitin-2. Relative prohibitin-2 expression (AVG ± SD) is shown for adult female (n = 8) and adult male (n = 10) L. salmonis from the drug susceptible (S) laboratory-maintained strain. The prohibitin-2 expression differed significantly between L. salmonis males and females (p = 0.0117, Mann-Whitney test).