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High-Throughput Sequencing of Islet-Infiltrating Memory CD4+ T Cells Reveals a Similar Pattern of TCR Vβ Usage in Prediabetic and Diabetic NOD Mice

Figure 4

High variation in TRBJ usage in islet-infiltrating CD4+CD44high T cell clonotypes.

The average frequency of TRBJ gene usage by all CD4+CD44high clonotypes (A) and high-frequency clonotypes (B) from prediabetic mice (**p = 0.0054 for TRBJ1-5*1) and diabetic mice. The average frequency of TRBJ gene usage by all CD4+CD44high clonotypes and high-frequency clonotypes from (C) prediabetic mice (**p = 0.0081 for TRBJ2-7) and (D) diabetic mice (*p = 0.019 for TRBJ1-1, *p = 0.04 for TRBJ2-3, and *p = 0.022 for TRBJ2-5). All data are the mean ± SEM.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0076546.g004