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The Bacterial Carbon-Fixing Organelle Is Formed by Shell Envelopment of Preassembled Cargo

Figure 5

A solvent-accessible dye pulse labels foci that subsequently divide, but do not dissipate.

(A) Montage showing one labeled RuBisCO focus partitioning into two or more daughter carboxysomes and persisting over the time interval of the experiment. Red: phase contrast. Green: RbcL-SNAP. Scale bar: 1µm. Time interval: 2 hours. (B) Intensity of the mother carboxysome (pink trace) sometimes decreases when new daughters (green and blue traces) are born. In other cases, the decrease is not detectable (grey trace). (C) Distribution of the number of new carboxysome foci formed per cell over the course of an experiment (20 hours). RuBisCO foci either persisted or divided over 20 hours (n = 220). All original foci were detectable at the end of the experiment.

Figure 5