Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

The Bacterial Carbon-Fixing Organelle Is Formed by Shell Envelopment of Preassembled Cargo

Figure 4

The carboxysome oxidizes over the course of its maturation.

(A) RbcL-roGFP1 excited with 410nm (left) and 488nm (middle) produces ratiometric (488nm/410nm) differences in emission (right). Scale bar: 1µm. (B) A histogram of this ratio measured at each carboxysome focus reveals an asymmetric distribution biased toward a relatively oxidized state. (C–F) Montages of RbcL-roGFP1 show transitions from predominantly 488nm excitation (green) to 410nm excitation (magenta) over the maturation period of carboxysomes. Carboxysomes establish an oxidizing state before the appearance of a new carboxysome, rarely reopening to the cytosol after an initial closure (G). Arrows indicate birth events. Scale bar: 1µm. Interval: 20 minutes.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0076127.g004