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Identification of an Enhancer That Increases miR-200b~200a~429 Gene Expression in Breast Cancer Cells

Figure 5

Over-expression of miR-200b eRNA has no effect on miR-200b~200a~429 promoter activity in epithelial and mesenchymal HMLE cells.

Schematic representation of the miR-200b~200a~429 luciferase reporter constructs, PRO (-321/+19), LOCUS (-5771/+19), PRO&ENH (-321/+19 to -5711/4607) and ENH (-5711/-4607). The miR-200b~200a~429 reporters, pcDNA3.1, pcDNA3.1 Zeb1, pcDNA3.1 Zeb2 or the pcDNA3.1 miR-200b eRNA plasmids, and the Renilla vector were co-transfected into epithelial HMLE (white bars) or mesenchymal HMLE (black bars) cells. Transiently transfected cells were incubated for approximately 24-48 hours. Data are normalized by Renilla luciferase activity and represent means ± SD of at least three independent experiments.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0075517.g005