Circadian Regulation of the Na+/K+-Atpase Alpha Subunit in the Visual System Is Mediated by the Pacemaker and by Retina Photoreceptors in Drosophila Melanogaster
Figure 6
Pattern of ATPα immunoreactivity in flies with reduced clock activity in photoreceptors ( gmr-GAL4>UAS-Δcyc) (A) and glia (repo-GAL4>UAS-Δcyc) (B).
The fluorescence index ± SE is shown as a function of time. (A) In gmr-GAL4>UAS-Δcyc flies immunofluorescence levels were high at ZT1, ZT13 and ZT16 and lowered by about 50% at ZT4. There were statistically significant differences between ZT4 and the other time points. (B) In repo-GAL4>UAS-Δcyc flies the immunofluorescence index was higher at ZT1 and ZT13 and lowered by 45.1% at ZT4 and by 56.4% at ZT16. There were statistically significant differences between ZT1 and ZT4, ZT1 and ZT16, ZT13 and ZT4, ZT13 and ZT16. Parametric ANOVA Tukey's test; p<0.05. The two stars symbols indicate statistically significant differences between the experimental strains and CantonS controls at different time points.