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PhagoSight: An Open-Source MATLAB® Package for the Analysis of Fluorescent Neutrophil and Macrophage Migration in a Zebrafish Model

Figure 8

Comparison of the segmentation and tracking results against ICY for one synthetic and one real data set.

In ICY, “size filter” was used with increasing values of size; PhagoSight input thresholds were automatically determined and then modified from 40% to 140% of the original. (a) Synthetic data set tracked with ICY, (b) Synthetic data set tracked with PhagoSight, (c) Real data set tracked with ICY, (d) Real data set tracked with PhagoSight. Solid line and circle markers corresponds to distance from the automatically generated tracks to the gold standard (DAG) and dotted line with square markers corresponds to the distance from the gold standard to the automatically generated tracks (DGA).

Figure 8

doi: https://doi.org/10.1371/journal.pone.0072636.g008