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PhagoSight: An Open-Source MATLAB® Package for the Analysis of Fluorescent Neutrophil and Macrophage Migration in a Zebrafish Model

Figure 2

Hysteresis thresholding segmentation of a single neutrophil at five time points.

The region described by low threshold (bright) will contain one or more regions described by the high threshold (dark); if segmented solely with a single high threshold several unconnected regions would arise. A single low threshold would in turn produce more regions of low intensity not shown here.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0072636.g002