Improved Bone Morphogenetic Protein-2 Retention in an Injectable Collagen Matrix Using Bifunctional Peptides
Biotinylated BMP-2 was immobilized on streptavidin-coated plates and subjected to multiple rounds of phage display selections using 10 different phage display peptide libraries. Individual BMP-binding phage were isolated and the sequence of the BMP-binding peptide deduced from the phage DNA sequence. Alignment of the peptides revealed two general sequence motifs among the peptides: motif 1: W-X-X-F-X-X-L and motif 2: L-X-F-P-L-K. These motifs were used to generate 2nd generation focused libraries. In addition, representative synthetic peptides were made and tested for binding to BMP-2.