ERK Positive Feedback Regulates a Widespread Network of Tyrosine Phosphorylation Sites across Canonical T Cell Signaling and Actin Cytoskeletal Proteins in Jurkat T Cells
Figure 7
Quantitative phosphoproteomic analysis of proteins associated with the KEGG TCR signaling pathway.
Depicted is a SILAC heatmap representation of temporal changes in tyrosine phosphorylation of proteins associated with the KEGG TCR signaling pathway. Heatmaps were calculated from the averages of four biological replicate experiments. SILAC ratios between U0126-treated and control DMSO-treated Jurkat T cells are represented for each phosphopeptide and time point. A white dot within the SILAC heatmaps indicate a statistically significant difference (q value <0.02) in the comparison between U0126-treated and control SILAC ratios for that time point. Abbreviations: GSK3 beta, Glycogen synthase kinase 3 beta; PI3K alpha, Phosphatidylinositol 3 kinase regulatory subunit, alpha; SHP-2, Protein tyrosine phosphatase, nonreceptor type 11.