Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae
Yeast expressing fusions of each of the indicated proteins to the C-terminus of Cdc12 were imaged to assess whether they perturb its function. Perturbation of Cdc12 function manifests as misshapen yeast cells and/or mislocalized Cdc12. The green fluorescent proteins show minimal perturbation; mKate2 and mKO2 show major perturbation; mTagBFP2 is intermediate. Brightness has been normalized separately for each image so it is not comparable from image to image.