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Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae

Figure 1

Schematic design of tagging plasmids.

The overall design of these plasmids is identical to the pFA6a-link tagging plasmids previously published [6]. yoFP is one of the 24 yeast optimized proteins cloned here and S.M. is the yeast selectable marker, either SpHis5, CaUra3, or KanR. These tagging sequences can be amplified with the forward primer (gene-specific sequence)-GGTGACGGTGCTGGTTTA and reverse primer (gene-specific sequence)-TCGATGAATTCGAGCTCG. A complete list of plasmids constructed in this study is in Table S2.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0067902.g001