Differential Expression of Three Members of the Multidomain Adhesion CCp Family in Babesia bigemina, Babesia bovis and Theileria equi
Figure 3
Immunoblot analysis demonstrating the expression of CCp1-3 by temperature-induced B. bigemina parasites.
Expression of CCp1-3 proteins was investigated in B. bigemina cultures incubated for 24 h at 28°C. Expression of CCp1 was detected only by extracellular sexual stages (Sex Stg) whereas CCp2 and CCp3 were detected in both Sex Stg and intracellular parasites (Intracell) populations. No bands with the expected molecular mass of the CCp proteins were detected in non-induced B. bigemina cultures (uCulture) or in uninfected red blood cells (uRBC). Polyclonal mouse anti-CCp1 peptide, rabbit anti-CCp2-peptides or CCp3-peptides, and bovine serum from an animal chronically infected with B. bigemina were used at a 1∶50 dilution. Anti-mouse, anti-rabbit, or anti-bovine HRP conjugates were used at a 1∶4,000 dilution. Black arrows indicate the expected size of CCp1-3.