Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells
a) Growth curves of E. coli cultures. A culture was grown for 24 hours, diluted 1000-fold, and allowed to grow again for 24 hours. Samples from the cultures were fixed for analysis at the colored time-points. Solid line is the fit to logistic growth model. b) The dry density of the culture by sampling time point. Technical replicates of these fixed samples show that the changes in density are reproducible and not attributable to instrument error. c) Probability distributions of dry mass, rescaled so that the modal mass had a density of one. Lines of the same color are technical replicates, measured several days apart.