Nodes Having a Major Influence to Break Cooperation Define a Novel Centrality Measure: Game Centrality
Prisoner’s dilemma game was simulated on a high-fidelity yeast interactome , and game centrality measures were calculated as described in Methods. 171 proteins out of the 2,444 nodes of the high-fidelity yeast interactome were selected by selecting nodes, which diminished the cooperation level from ∼1 to 0.9 or below. Functional analysis of the 171 proteins was performed using the Cytoscape plug-in, BiNGO  to assess the over-representation of associated Gene Ontology molecular function terms. Gene Ontology Slim definitions for Saccharomyces cerevisiae  were used discarding the evidence codes IEA (inferred from electronic annotation), ISS (inferred from sequence structural similarity) and NAS (non-traceable author statement). A hypergeometric test with false discovery rate correction  was used to select and visualize the significantly enriched GO functions at a level p<0.001, using the GO-s of the entire network as reference set. Colors represent functional categories: red, nucleus-related; blue, transport-related; green, signaling-related; yellow denotes other functions. The size of the circles represents the number of proteins found in the category.