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Glycogen and Glucose Metabolism Are Essential for Early Embryonic Development of the Red Flour Beetle Tribolium castaneum

Figure 8

Tc-GSK-3 knockdown affects T. castaneum egg laying, larvae hatching and glycogen content.

(A) Tc-GSK-3 expression decreases after Tc-GSK-3 dsRNA injection. (B) Number of laid eggs diminishes 50% after Tc-GSK-3 RNAi when compared to the control. (C) Number of hatching larvae decreases after Tc-GSK-3 RNAi to about 20% of the control. (D) Glycogen content increases in Tc-GSK-3 RNAi eggs when compared to the control (LacZ RNAi eggs). Asterisk indicates that the difference between the two groups is statistically significant (p<0,05).

Figure 8

doi: https://doi.org/10.1371/journal.pone.0065125.g008