Browse Subject Areas

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

Figure 1

Illustration of supercharged GFP surfaces.

The GFP backbone is shown in green cartoon, Asp/Glu side chains are shown in red spheres, Arg/Lys side chains are shown in blue spheres. Left: mutations in negatively-supercharged variants. Center: wild-type superfolding GFP. Right: mutations in positively-supercharged variants.

Figure 1