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Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

Figure 1

Illustration of supercharged GFP surfaces.

The GFP backbone is shown in green cartoon, Asp/Glu side chains are shown in red spheres, Arg/Lys side chains are shown in blue spheres. Left: mutations in negatively-supercharged variants. Center: wild-type superfolding GFP. Right: mutations in positively-supercharged variants.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0064363.g001