Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability
The GFP backbone is shown in green cartoon, Asp/Glu side chains are shown in red spheres, Arg/Lys side chains are shown in blue spheres. Left: mutations in negatively-supercharged variants. Center: wild-type superfolding GFP. Right: mutations in positively-supercharged variants.