A Prospective Study among Patients Presenting at the General Practitioner with a Tick Bite or Erythema Migrans in the Netherlands
Seropositive results of the C6 ELISA*, the IgM** and IgG*** immunoblot for Borrelia-specific antibodies in simultaneously tested paired serum samples, collected at enrolment and at follow-up after three months.
* The C6 ELISA test was considered positive if the ELISA index scored ≥1.10.** The IgM immunoblot was considered positive if there was reactivity to the 22 kDa band, together with at least one of the following specific bands: 15–20, 22, 30–39, and 41 kDa.*** The IgG immunoblot was considered positive if at least four reactive bands were present including at least one of the following specific bands: 17, 22, 39, 58 and 92 kDa.