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CLUSTOM: A Novel Method for Clustering 16S rRNA Next Generation Sequences by Overlap Minimization

Figure 4

Taxonomy-based accuracy evaluation.

The clustering results of CLUSTOM, NW-DOTUR-AL, ESPRIT-Tree, NAST-mothur-AL and NW-mothur-AL were evaluated using the 16S–SILVA sequences. (A–B): The number of clustered OTUs was examined at the species (3% dissimilarity; A) and genus (5%; B) levels for every OTU size (number of sequences in a cluster). (C–D): The precision and recall statistics as well as their harmonic mean (F2) [25] were used to evaluate clustering results of the five programs at the two taxonomic levels.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0062623.g004