A 43-kDa TDP-43 Species Is Present in Aggregates Associated with Frontotemporal Lobar Degeneration
Figure 4
Protein components of the aggregate and monomer regions of SDD-AGE gels.
(A) Proteins concentrated from aggregate or monomer regions of an FTLD case compared. Silver stained SDS-PAGE gel with equal volumes of a 40-fold dilution of the monomer region concentrate (M) and undiluted aggregate region concentrate (A) are loaded on the gel. These fractions comprise different proteins. Semi-quantitative analysis indicates the total protein content of the aggregate fraction is approximately 1% that of the monomer fraction. Bars on the left indicate the migration of molecular weight protein standards of the depicted masses (kDa). (B) Comparison of proteins concentrated from the aggregate region of SDD-AGE gels for two FTLD cases and a control (the volume loaded is ∼22% of the concentrate from a region such as in figure 3a). The lane labeled W contains approximately 1 µg (total protein) from a whole brain homogenate for comparison. The aggregate regions of either FTLD cases or controls comprise a complex mixture of proteins. No consistent difference in the band pattern is seen between cases and controls. The TDP-43 bands seen in immunoblots of proteins from the aggregate region of FTLD cases are not distinctly seen in these silver stained gels.