High Resolution Helium Ion Scanning Microscopy of the Rat Kidney
Glomerular podocyte slit diaphragms from the same kidney as shown by HIM in Fig. 2, imaged by conventional scanning electron microscopy (SEM).
(A) Sample imaged without sputter coating, using an in-lens detector. (B, C) Coated samples imaged using either the standard SE2 detector (B) or an in-lens detector (C). Structural details of the slit diaphragm are less well defined than in the HIM image shown in Fig. 2B. Bar = 100 nm.