OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects
A qualitative assessment of the versatility of OpenCFU was undertaken by analysing pictures of different circular biological objects: a clear (A) and a poor quality (B) picture of Staphylococcus aureus colonies, a low-contrasted picture of Escherichia coli (C), a noisy picture of mustard seeds (D), a noisy picture of soy-bean seeds (E), and a micrography of Carduus sp. pollen (F). For the purpose of explanation, only representative areas (200200 pixels) of six processed images are shown here. Original portions of images are on the left and correspond to the graphical results obtained using OpenCFU on the right.